Full Title:Fundamental Microbiology
Module Code:BIOL S7009
Credits: 7.5
Valid From:Semester 1 - 2013/14 ( September 2013 )
Module Delivered in 4 programme(s)
Module Description:The student will apply their knowledge of the bacterial cell and basic microbiology to the study of microbial nutrition, ecology, growth, and tools of the microbiology laboratory.
Learning Outcomes:
On successful completion of this module the learner should be able to
  1. Recall the generalized anatomy of bacterial cells.
  2. Outline the principles of microbial population growth and apply these to the enumeration of microorganisms using direct and indirect quantification methods.
  3. Discuss the major environmental factors to which microbes must adapt for survival.
  4. Describe the purpose and processes of selected procedures used in handling, maintaining, and studying microorganisms.
  5. Perform basic microbiology laboratory procedures for the growth, maintenance and identification of an unknown microorganism.

Module Content & Assessment

Indicative Content
Topic 1: Proteobacteria
Revision of microbial morphology and cytology.
Topic 2: Microbial Growth
Bacterial cell division, growth of bacterial populations, generation time calculations, direct and indirect quantification methodologies: ACC plate counts, microscopic counts, MPN techniques, membrane filtration, turbidity and standard curves.
Topic 3: Microbial Nutrition and Ecology
Impact of nutrient availability, temperature, pH, gas content and osmotic pressure in the environment of microorganisms and resulting microbial adaption, associations and classification.
Topic 4: Tools of the Microbiology Laboratory
Laboratory culture and identification of microorganisms: laboratory methods for studying microorganisms: inoculation, incubation, isolation, inspection, information gathering and interpretation, identification.
1. Laboratory health and safety and GLP. 2. Media preparation, inoculation and aseptic techniques 3. Microbial staining and microscopic examination 4. Culture techniques and characterisation 5. Selective and differential media 6. Investigation of physical and chemical factors that affect microbial growth 7. Determination of metabolic characteristics of microorganisms including carbohydrate, protein and lipid catabolism. 8. Rapid methods of identification of microorganisms 9. Enumeration of microorganisms from a variety of samples using a variety of techniques.
Teaching and Learning Strategy
Lectures: Blended and flexible learning will be used throughout. This will take the form of face to face interactive lecture sessions complemented by online resources (reusable learning objects and the National Digital Learning Repository) and online interactive activities (learn smart study assignments). Students will be directed to relevant sections of appropriate text to reinforce material covered in lectures. Online demonstrations will illustrate key concepts of the course and will be available to students via the VLE throughout the year. Practical exercises are designed to enable students to develop the required technical competencies, attitudes and behaviours in microbiology.
Assessment Breakdown%
Course Work25.00%
End of Module Formal Examination50.00%

Full Time

Course Work
Assessment Type Assessment Description Outcome addressed % of total Marks Out Of Pass Marks Assessment Date Duration
Class Test Students will complete an interactive on-line exam consisting of a series of multiple choice, true and false, fill in the blank, short answer questions, diagram matching and labelling. Students can prepare for this exam by accessing specific interactive on-line study modules and quizzes. 1 10.00 0 0 Week 5 0
Class Test Students will select and apply appropriate equations to complete a series of mathematical calculations for the enumeration of microorganisms. 2 15.00 0 0 Week 10 0
No Project
Assessment Type Assessment Description Outcome addressed % of total Marks Out Of Pass Marks Assessment Date Duration
Practical/Skills Evaluation Students will complete result report sheets during the practical class that are specifically designed to develop observation skills and to promote accurate data recording and subsequent interpretation. Additionally the student will be provided with an unknown bacterium which they will maintain and test throughout the semester. They must collate this data and submit an identification report. Completion of interactive virtual labs will reinforce key concepts. 3,4,5 25.00 0 0 n/a 0
End of Module Formal Examination
Assessment Type Assessment Description Outcome addressed % of total Marks Out Of Pass Marks Assessment Date Duration
Formal Exam End-of-Semester Final Examination 2,3,4 50.00 0 0 End-of-Semester 0

DKIT reserves the right to alter the nature and timings of assessment


Module Workload & Resources

Workload: Full Time
Workload Type Workload Description Hours Frequency Average Weekly Learner Workload
Lecture No Description 3.00 Every Week 3.00
Practical No Description 3.00 Every Week 3.00
Directed Reading No Description 2.00 Every Week 2.00
Independent Study No Description 3.00 Every Week 3.00
Total Weekly Learner Workload 11.00
Total Weekly Contact Hours 6.00
This course has no Part Time workload.
Recommended Book Resources
  • Talaro, K. P. 2011, Foundations in Micobiology: Basic Principles, 8th Ed., McGraw-Hill [ISBN: 978-0071316736]
  • Sherlock Orla 2013, Foundation Microbiology Laboratory Manual, DkIT.
Supplementary Book Resources
  • Bauman 2012, Microbiology with Diseases by Taxonomy, 2nd Ed., Pearson [ISBN: 978-0321667663]]
  • Madigan, Martinko, Dunlap, Clark, Brock Biology of Microorganisms, 12th Ed., Pearson [ISBN: 978-0132324601]
This module does not have any article/paper resources
Other Resources

Module Delivered in

Programme Code Programme Semester Delivery
DK_SENVI_8 Bachelor of Science (Honours) in Environmental Bioscience 3 Mandatory
DK_SAPBI_7 Bachelor of Science in Applied Bioscience 3 Mandatory
659 Bachelor of Science in Environmental Bioscience 3 Mandatory
Dk_SAPBI_6 Higher Certificate in Science 3 Mandatory