Full Title:Microbiology and Biochemistry
Module Code:BIOL S7010
Credits: 5
Valid From:Semester 1 - 2013/14 ( September 2013 )
Module Delivered in 1 programme(s)
Module Description:This practical focused module aims to equip students with the necessary skills to carry out basic microbiological diagnostic procedures in clinical practice. The student will be able to demonstrate knowledge of microbial physiology, pathogens and their identification, infection and disease processes. Additionally the necessary biochemical material to support modules in physiology, nutrition and pathology will be studied.
Learning Outcomes:
On successful completion of this module the learner should be able to
  1. Describe the purpose and processes of the primary procedures that are used in handling, maintaining, characterising and identifying microorganisms in veterinary microbiology.
  2. Outline the role of microbes in infection development and disease production.
  3. List and describe principal bacterial pathogens of veterinary interest.
  4. Complete routine veterinary microbiology diagnostic procedures and accurately report results.
  5. Summarise the major steps in the cellular metabolism of proteins, fats and carbohydrates and state the function of important biomolecules relating function to structure.
  6. Describe the properties of enzymes and their mechanism of action.

Module Content & Assessment

Indicative Content
Topic 1: Prokaryotes -the Proteobacteria
Bacterial cell external structures, envelope, internal structures and cell morphology.
Topic 2: Laboratory culture and identification of microorganisms
Aseptic technique, inoculation, incubation, isolation, inspection, information gathering and interpretation, identification.
Topic 3: Specimen collection for microbiological evaluations.
Guidelines for specimen collection, handling, processing and storage. Safe dispatching of samples to contract laboratories.
Topic 4: Antimicrobial testing in veterinary practice
Features of antimicrobial drugs, mode of action of antimicrobials, indications for susceptibility testing, Beta-lactamase and agar diffusion susceptibility methods.
Topic 5: Microbial infections and diseases caused by various animal pathogens
Animal microbiota, factors in the development of a disease, sources and transmission of microbes, immune response to infection. Bacterial pathogens focusing on Staphylococci species, Enterobacteriaciae species, Pseudomonas species, Burkholderia species.
Microbiology Laboratory Practicals
1. Demonstration of hand hygiene and microbiological evaluation of procedure. 2. Collection and handling of specimens and safe disposal of microbiology specimens. 3. Processing specimens for staining and microscopic examination. Staining of microbiological specimens. 4. Aseptic technique, basic media preparation, inoculation of agar plates, colony morphology evaluations. 5. Plating swabs from clinical samples. Selective and differential plating. 6. Techniques for preliminary identification of microorganisms. 7. Antimicrobial susceptibility testing via disc diffusion and E-tests
Topic 6: Proteins
Functions of Proteins in Cells (Structure and Support; Protection; Movement; Catalysts; Hormones; Transport). Classification of Proteins (Fibrous, Globular, Simple, Conjugated). Amino acids (essential and non-essential) and Protein Structure (Primary, Secondary, Tertiary, Quaternary).
Topic 7: Enzymes
Enzyme catalysis, Active site, Substrate, Enzyme Classification, Co-enzymes and Co-factors, Sensitivity of enzymes (pH and temperature), Competitive and non-competitive inhibition, Examples of enzymes used for the diagnosis of disease in animals.
Topic 8: Lipids
Structure of lipids, Fatty acids (saturated versus unsaturated), Essential fatty acids and deficiency in animals, Derivatives of Essential Fatty acids (prostacyclins, prostaglandins, tromboxanes, leucotrienes).
Topic 9: Carbohydrates
Function of carbohydrates (simple, storage, structural). Structure of carbohydrates (monosaccharides, oligosaccharides, polysaccharides).
Topic 10: Metabolism
Catabolism versus anabolism. Fat metabolism (beta oxidation,ketone,ketosis). protein metabolism (transamination and deamination). Aerobic Carbohydrate Metabolism (Glycolysis, Krebs/Citric Acid cycle. Electron Transport Chain. Adenosine triphospate (ATP) and functions of ATP. Anaerobic Carbohydrate Metabolism - Glycolysis, Lacticacidosis. Gluconeogenesis, Cori Cycle.
Teaching and Learning Strategy
Blended and flexible learning will be used throughout. This will take the form of face to face interactive lecture sessions complemented by online resources (animations, videos, virtual labs, microbiology discussion forum) and online activities (vet logic, interactive quizzes).
Assessment Breakdown%
Course Work50.00%
End of Module Formal Examination50.00%

Full Time

Course Work
Assessment Type Assessment Description Outcome addressed % of total Marks Out Of Pass Marks Assessment Date Duration
Class Test The class exam will consist of a series of multiple choice, true and false, fill in the blank, short answer questions and diagram labelling. 1,5,6 10.00 0 0 Week 8 0
Practical/Skills Evaluation Students will process an unknown microbiology specimen as encountered in practice and will complete a preliminary identification and antimicrobial susceptibility profile for their isolate. 1,4 15.00 0 0 Week 13 0
Practical/Skills Evaluation Students will complete result report sheets during the practical class that are specifically designed to develop observation skills and to promote accurate data recording and subsequent interpretation in-line with veterinary diagnostics. Practical skills and competencies will be assessed via performance evaluation in class. Completion of interactive virtual labs will reinforce key concepts. 1,4 25.00 0 0 Every Week 0
No Project
No Practical
End of Module Formal Examination
Assessment Type Assessment Description Outcome addressed % of total Marks Out Of Pass Marks Assessment Date Duration
Formal Exam End-of-Semester Final Examination 6,5,1,3,2 50.00 0 0 End-of-Semester 0
Reassessment Requirement
A repeat examination
Reassessment of this module will consist of a repeat examination. It is possible that there will also be a requirement to be reassessed in a coursework element.

DKIT reserves the right to alter the nature and timings of assessment


Module Workload & Resources

Workload: Full Time
Workload Type Workload Description Hours Frequency Average Weekly Learner Workload
Lecture No Description 5.00 Every Week 5.00
Practical No Description 2.00 Every Week 2.00
Directed Reading No Description 1.00 Every Week 1.00
Independent Study No Description 2.00 Every Week 2.00
Total Weekly Learner Workload 10.00
Total Weekly Contact Hours 7.00
This course has no Part Time workload.
Recommended Book Resources
  • Quinn, Markey 2003, Concise Review of Veterinary Microbiology, 1st Ed., Wiley-Blackwell [ISBN: 978-1-4051-0898-0]
  • Wortinger, A 2007, Nutrition for Veterinary Technicians and Nurses, 1st Ed., Wiley [ISBN: 9780813829135]
Supplementary Book Resources
  • Nelson,D and Cox,M 2009, Lehninger Principles of Biochemistry, 5th Ed., Freeman [ISBN: 9780716771081]
  • P. J. Quinn, B. K. Markey, F. C. Leonard, P. Hartigan, S. Fanning, E. S. FitzPatrick 2011, Veterinary Microbiology and Microbial Disease, 2nd Ed., Wiley-Blackwell [ISBN: 978-1405158237]
  • Joanna M. Bassert VMD , Dennis M. McCurnin DVM MS Dipl ACVS, McCurnin's Clinical Textbook for Veterinary Technicians, 7th Ed., Ch 18, Elsevier [ISBN: 978-1-4160-5700-0]
  • Carter, G.R., Wise, D.J. 2004, Essentials of Veterinary Bacteriology and Mycology, 6th Ed., Iowa State Press [ISBN: 9780813811796]
  • Lodish et al, Molecular Cell Biology, 5th Edition Ed., Freeman [ISBN: 0716743663]
This module does not have any article/paper resources
Other Resources

Module Delivered in

Programme Code Programme Semester Delivery
Dk_SVETN_7 Bachelor of Science in Veterinary Nursing 2 Mandatory