Full Title:Applied Microbiology
Module Code:MCBL S7001
Credits: 7.5
Valid From:Semester 1 - 2013/14 ( September 2013 )
Module Delivered in 3 programme(s)
Module Description:This module aims to provide the student with the essential features of microbiology relevant to the food and pharmaceutical industries. Students will also be introduced to microbial ecosystems and methods in microbial ecology.
Learning Outcomes:
On successful completion of this module the learner should be able to
  1. Classify types of foodborne disease providing examples and identify the major methods of food preservation used to avert foodborne disease.
  2. Identify the major microorganisms of concern in drinking water and water used for commercial and recreational purposes, and summarise the primary methods of assessing microbiological quality of water.
  3. Discuss microbial contamination of pharmaceutical products, select control strategies applicable for pharmaceutical manufacture and describe how these methods can be evaluated.
  4. Describe the structure and microbial content of soil and the activities of rhizosphere microorganisms.
  5. Select and apply appropriate laboratory procedures for the microbiological examination of various products, demonstrating whether that product meets the appropriate microbiological production and safety criteria and is compliant with legislation.

Module Content & Assessment

Indicative Content
Topic 1: Food Microbiology
Food ecology, food preservation, food spoilage, foodborne disease: foodborne infection- Salmonella and Campylobacter; foodborne intoxication-Staphylococcus aureus, Clostridium; foodborne toxicoinfection – Bacillus. Food legislation.
Topic 2: Public Health and Water Quality
Enterobacteriaceae, Coliforms, Escherichia coli, Indicator organisms, Water borne diseases, Microbiological examination of water, Legislation.
Topic 3: Pharmaceutical Microbiology
Microbial product contamination, biocides as antiseptics, disinfectants and preservatives. Sterile products, sterilisation processes, bioburden and sterility assurance. European Pharmacoepia, microbiological legislation.
Topic 4: Microbial Ecology
Microbial ecology/diversity, Microbial communities and ecosystems, Community organisation and interactions – biofilm, bioaggregates. Microorganisms in terrestrial ecosystems. Microorganisms in marine and fresh water systems; Methods in microbial ecology.
1. Preparation of food and pharmaceutical products for microbiological analysis 2. Recovery of stressed/metabolically injured and viable non cultural microorganisms. 3. Identification and quantification of microbes in water, ACC, Indicator organisms and MPN technique, Membrane filtration methods, Quantitray. 4. Qualitative and quantitative examination of food and pharmaceutical products 5. Sterility assurance including bioburden determination, environmental monitoring, validation of sterilisation procedures and sterility testing in accordance with European pharmacopoeia. 7. Evaluation of biocides via suspension tests, use dilution tests, disc diffusion test and phenol coefficient tests. 8. Endotoxin testing, pyrogen testing. 9. Analysis of soil 10. Determination of biofilm capabilities of soil isolates. 11. Selective enrichment of soil samples for xenobiotic degrading microorganisms
Teaching and Learning Strategy
Blended and flexible learning will be used throughout. This will take the form of face to face interactive lecture sessions complemented by various online resources. Group and peer learning will be facilitated in and out of class via use of discussion forums, wiki's blogs etc.
Assessment Breakdown%
Course Work50.00%
End of Module Formal Examination50.00%

Full Time

Course Work
Assessment Type Assessment Description Outcome addressed % of total Marks Out Of Pass Marks Assessment Date Duration
Project Conduct research and produce a report describing the microbiology of a specific food group including sources of microorganisms, normal microbiological quality, spoilage and suggested control mechanism, pathogens associated, suggested laboratory analysis and any relevant legislation. 1,2,5 20.00 0 0 Week 8 0
Written Report Students will complete microbiological analysis of food, pharmaceutical and ecology specimens in accordance with relevant legislation. Written reports demonstrating accurate results reporting, data interpretation and analysis will be completed. 1,2,3,4,5 30.00 0 0 Every Week 0
No Project
No Practical
End of Module Formal Examination
Assessment Type Assessment Description Outcome addressed % of total Marks Out Of Pass Marks Assessment Date Duration
Formal Exam End-of-Semester Final Examination 1,3,2,4 50.00 0 0 End-of-Semester 0

DKIT reserves the right to alter the nature and timings of assessment


Module Workload & Resources

Workload: Full Time
Workload Type Workload Description Hours Frequency Average Weekly Learner Workload
Lecture No Description 3.00 Every Week 3.00
Practical No Description 3.00 Every Week 3.00
Directed Reading No Description 2.00 Every Week 2.00
Independent Study No Description 4.00 Every Week 4.00
Total Weekly Learner Workload 12.00
Total Weekly Contact Hours 6.00
This course has no Part Time workload.
Recommended Book Resources
  • Madigan, M. T. Martinko, J. M. & Parker, J. Brock 2008, Brock: Biology of Microorganisms., 12th Ed., Prentice Hall International Inc, London [ISBN: 978-0132324601]
  • Ray, B., Bhuna, A. 2007, Fundamental Food Microbiology, 4th Ed., CRC Press [ISBN: 978-0849375293]
  • McLandsborough, L. 2003, Food Microbiology Laboratory, 1st Ed., CRC Press [ISBN: 978-0849312670]
  • Hanlon, G., Hodges, N. 2013, Essential Microbiology for Pharmaceutical Science, 1st Ed., Wiley-Blackwell [ISBN: 978-0470665343]
  • Orla Sherlock 2012, Applied Microbiology Laboratory Manual, DkIT
Recommended Article/Paper Resources
Other Resources

Module Delivered in

Programme Code Programme Semester Delivery
DK_SENVI_8 Bachelor of Science (Honours) in Environmental Bioscience 5 Mandatory
DK_SAPBI_7 Bachelor of Science in Applied Bioscience 5 Mandatory
659 Bachelor of Science in Environmental Bioscience 5 Mandatory