Full Title:Advanced Analytical Science
Module Code:INST S7Z03
Credits: 7.5
Valid From:Semester 1 - 2015/16 ( September 2015 )
Module Delivered in No Programmes
Module Description:The aims of this module are to • introduce and survey the applicability of novel and emerging spectroscopic, mass spectrometry, flow cytometry, capillary electrophoresis and nanoscience techniques. • increase student’s confidence in undertaking biochemical and chemical analysis, to work independently and in a research team, to become independent in designing and executing experiments and to provide good quality presentations of their findings.
Learning Outcomes:
On successful completion of this module the learner should be able to
  1. Describe the fundamental theoretical basis of infra-red spectroscopy and mass spectrometry for chemical and biochemical analysis and describe the principles behind capillary zone electrophoresis in its basic form and with a few selected important variants.
  2. Recognise and be able to discuss the principles, advantages and basic operations of flow cytometry as a bioanalytical technique.
  3. Evaluate the basic approaches in nanoscience to materials, fabrication and experimental techniques on the nanoscale.
  4. Demonstrate proficiency in using a range of analytical instrumentation and techniques and report and present data electronically using an Electronic Laboratory Notebook.
  5. Combine technical skills and theoretical knowledge to define a problem in analytical science and select and apply the most suitable technique to solve the problem while working as a group.

Module Content & Assessment

Indicative Content
Infra Red
The interaction of infrared radiation with molecules; the infrared spectrum and its interpretation. Key features of IR instruments including sample preparation.
Mass Spectrometry
Methods of ion production in mass spectrometry, the generation of MS spectra and their interpretation. GCMS and LCMS hyphenated techniques
Capillary Electrophoresis
The general principles of capillary zone electrophoresis including sample addition, detection of separated components and the electro-endosmotic effect and its consequences; useful additives to the separation medium and their specific applications
Flow Cytometry
Principle of operation of flow cytometer, instrumentation, fluorescent detection, forward and side light scatter, flow cells, data interpretation, advantages, limitations and analytical capabilities.
Fundamental principles of nanotechnology, importance of size, properties of nanoparticles, carbon nanostructures, quantum dots, medical applications of nanotechnology.
Learning and Teaching Methods
Teaching methods will comprise delivery of lectures and practical sessions with an emphasis on deep learning in a student-centred learning approach. A variety of blended and eLearning techniques will be deployed including in-class demonstrations, problem-based learning, peer assisted learning, self assessment and use of multi-media (animations, videos, podcasts, eAssessments, virtual eLabs).
Virtual Learning Environment
The DkIT Virtual Learning Environment (Moodle) page for Analytical Instrumentation and Techniques 1 and 2 will be used extensively as a repository for lecture material, past exam papers, video links, online resource links, online quizzes, feedback, peer-reviewed articles as well as documents pertaining to practical lab sessions (Material Safety Data Sheets).
Electronic Laboratory Notebook
Students will be introduced to an emerging tool in industry for lab data and documentation management which will be used for reporting laboratory practicals in place of the standard hard copy notebook. The ELN is a web-based product enabling the user to easily create, store, share and manage their research data. Students will be able to store and retrieve any type of documents, including Images, GraphPad Prism and MS-Office, enter rich text, spreadsheets, mathematical formulae, chemical structures, share entries or entire notebooks in small group work, capture electronic "signatures" for approvals. The use of the ELN is inherently environmentally friendly as it replaces traditionally paper-intensive documentation and storage and adopting the ELN represents a sustainable approach to reporting (operates on all platforms including iPhone/smartphone).
Laboratory Practical Sessions
The following list is designed to serve as an illustration of possible practical exercises which would illustrate key concepts and techniques: Investigation of aspects of infra-red spectroscopy, Analysis of liquids by IR using NaCl plates, Preparation of a Nujol Mull for IR Analysis, Investigation of reproducibility of film technique in IR, Qualitative IR for isomers, Quantitative determination using Capillary Electrophoresis, Improving CE separation, Comparison of CE and HPLC techniques for drug analysis.
Assessment Breakdown%
Course Work50.00%
End of Module Formal Examination50.00%

Full Time

Course Work
Assessment Type Assessment Description Outcome addressed % of total Marks Out Of Pass Marks Assessment Date Duration
Practical/Skills Evaluation A programme of weekly 3-hour laboratory practical sessions will run concurrently with lectures to reinforce fundamental theoretical concepts. Students will perform common analytical techniques using a suite of analytical instrumentation by following basic operating procedures, thereby gaining hands-on experience. Each student will be required to write a formal laboratory report for each experiment using an Electron Laboratory Notebook (ELN). 1,2,4 20.00 0 0 Every Week 0
Project Using the problem-based learning technique, students (small group work) will be given a problem to solve in forensic/pharmaceutical/environmental science for which they should combine technical skills (developed in practical sessions) and theoretical knowledge (developed through classroom and self-directed learning) to define the problem and select and apply the most suitable technique(s) to solve the problem and produce an electronic report using the ELN. 1,2,3,4,5 20.00 0 0 Week 27 0
Continuous Assessment In class written evaluation of flow cytometry data from published articles or pre-acquired data. 1,2,3 10.00 0 0 Week 29 0
No Project
No Practical
End of Module Formal Examination
Assessment Type Assessment Description Outcome addressed % of total Marks Out Of Pass Marks Assessment Date Duration
Formal Exam End-of-Semester Final Examination 1,2,3 50.00 0 0 End-of-Semester 0

DKIT reserves the right to alter the nature and timings of assessment


Module Workload & Resources

Workload: Full Time
Workload Type Workload Description Hours Frequency Average Weekly Learner Workload
Lecture Learning and Teaching Methods described in Module Content 3.00 Every Week 3.00
Practical Weekly 3-hour laboratory practical sessions outlined in the Indicative Content section under Laboratory Practical Sessions 3.00 Every Week 3.00
Directed Reading Lecture notes, Peer-reviewed papers, Textbooks, e-Resources 3.00 Every Week 3.00
Independent Study Independent/Group study 3.50 Every Week 3.50
Total Weekly Learner Workload 12.50
Total Weekly Contact Hours 6.00
This course has no Part Time workload.
Recommended Book Resources
  • Harris D C 2009, Exploring chemical analysis, 4th Ed., WH Freeman
  • Skoog D.A., Holler F.J. and Crouch S.R. 2007, Principles of instrumental analysis, 6th Ed., Thomson Publ
  • Wilson K. and Walker J. 2005, Principles and techniques of biochemistry and molecular biology, 6th Ed., Cambridge Univ. Press
  • Harris D.C. 2007, Quantitative chemical analysis, 7th Ed., Freeman
  • Kealey D and Haines P J 2002, Instant notes in chemical analysis, Garland Science
  • Watson D.G. 2005, Pharmaceutical analysis, 2nd Ed., Elsevier
  • Stuart, Barbara 2004, Infrared spectroscopy fundamentals and applications [online resource], Wiley DkIT Ebrary Collection
  • Rolf Ekman et al 2009, Mass spectrometry [electronic resource] : instrumentation, interpretation, and applications, Wiley DkIT Ebrary Collection
  • Lee, Mike S. Zhu, Mingshe 2011, Mass Spectrometry in Drug Metabolism and Disposition : Basic Principles and Applications [online resource], Wiley DkIT Ebrary Collection
  • Sklar, Larry A. 2005, Flow Cytometry for Biotechnology [online resource], Oxford University Press DkIT Ebrary Collection
  • Binns, C. 2010, Introduction to Nanoscience and Nanotechnology [online resource], Wiley DkIT Ebrary Collection
Supplementary Book Resources
  • Stuart, Barbara. 1996, Modern infrared spectroscopy, Wiley
  • Smith, Brian C. 1999, Infrared spectral interpretation : a systematic approach, CRC Press
  • Luigi Mondello 2011, Comprehensive chromatography in combination with mass spectrometry [online resource], Wiley DkIT Ebrary Collection
  • Grady Hanrahan, Frank A. Gomez. 2009, Chemometric methods in capillary electrophoresis [online resource], Wiley DkIT Ebrary Collection
  • Hannon-Fletcher, Mary 2009, Advanced Techniques in Diagnostic Cellular Pathology [onine resource], Wiley DkIT Ebrary Collection
This module does not have any article/paper resources
Other Resources