Full Title:Analytical Science
Module Code:INST S7Z02
 
Credits: 7.5
Valid From:Semester 1 - 2019/20 ( June 2019 )
Module Delivered in 9 programme(s)
Module Description:The aims of this module are to: introduce students to selected aspects of modern chemical and biochemical analytical instrumentation and techniques; increase students' confidence in undertaking analysis (independently and in a team) and in designing and executing experiments; encourage students to take careful measurements to assess the validity of their results.
Learning Outcomes:
On successful completion of this module the learner should be able to
  1. Describe the fundamental theoretical basis of some chromatographic and spectroscopic including their applications and limitations.
  2. Compare and contrast common analytical techniques in separation science (GC and HPLC) and spectroscopy (Atomic and Molecular).
  3. Demonstrate proficiency in using a range of analytical instrumentation and techniques by following standard operating procedures.
  4. Combine technical skills and theoretical knowledge to define a problem in analytical science and select the most suitable technique to solve the problem.
  5. Assess the validity of analytical procedures and assays with reference to the ICH Harmonised Tripartite Guidelines on assay validation.
 

Module Content & Assessment

Indicative Content
Chromatography
Introduction and general principles of chromatography, modes including Adsorption, Partition, Ion Exchange and Size Exclusion. Focus on gas liquid chromatography, including instrumentation, mobile/stationary phase selection, packed/capillary columns, detectors (FID, MS), applications and associated problems. Focus on HPLC; overview of HPLC, normal/reverse phase, column/mobile phase selection, diode-array detection and applications. Comparison of GC and HPLC. Chromatographic Parameters; Efficiency defined mathematically, Resolution defined and its dependence on chromatographic parameters will be illustrated for optimisation of resolution.
Molecular Spectroscopy
The electromagnetic spectrum, absorption of UV/visible radiation, excitation of electrons, relationship of structure to absorption, Beer-Lambert Law. The range of colorimeters and spectrophotometers and a survey of the main components parts. Applications of UV and visible spectroscopy and quantitative calculations.
Atomic Spectroscopy
Principle of absorption of energy by ground state atoms in gaseous state, electronic transitions, absorption/emission. Flame-based analytical systems; instrumentation, processes involved and examples of applications. Plasma-based techniques (ICP); comparison with flame-based techniques. Spectral, molecular, ionization and physical interferences. Quantitative techniques (including standard addition, internal standard). Application of atomic spectroscopy for sustainable environmental monitoring.
Validation of Analytical Procedures/Assays
Focus on ICH (International Conference on Harmonisation) Harmonised Tripartite Guidelines on assay validation, validation characteristics; accuracy, precision, specificity, detection limit, quantitation limit, linearity, range will be defined and equations will be used in practice to validate data obtained by students during laboratory practicals and (where available).
Learning and Teaching Methods
Teaching methods will comprise delivery of lectures and practical sessions with an emphasis on deep learning in a student-centred learning. A variety of blended and eLearning techniques will be deployed including in-class demonstrations, classroom assessment techniques, problem-based learning, peer assisted learning, self assessment and use of multi-media (animations, videos, eAssessments).
Virtual Learning Environment
The DkIT Virtual Learning Environment (Moodle) page for Analytical Science will be used extensively as a repository for lecture material, past exam papers, video links, online resource links, online quizzes, feedback, peer-reviewed articles as well as documents pertaining to practical lab sessions (Material Safety Data Sheets).
Virtual Laboratory Experiments
The use of virtual laboratory experiments in chromatography, spectroscopy and immobilisation reaction systems will complement laboratory practical sessions and reinforce fundamental theoretical concepts (for example; students will gain access to a CHROMacademy account managed by lecturer for full access to chromatography database, including HPLC, GC and hyphenated techniques, eLabs, assessments, webcasts, tutorials, lab simulations/ tools, peer-reviewed technical articles and application notes. Certification by CHROMacademy is also available to students upon completion of assessments).
Laboratory Practical Sessions
The following list is designed to serve as an illustration of possible practical exercises which would illustrate key concepts and techniques: • Separation of pigments by thin layer chromatography, • Use of gas liquid chromatography to identify and quantify components in a mixture, • Use of internal and external standards in gas liquid chromatography for quantification, • Use of high performance liquid chromatography to detect and quantify selected analytes, • To assess column quality in HPLC separations, • To verify Beer-Lambert Law in UV-Vis spectroscopy, • To study absorption of visible light by compounds and their absorption spectra, • To study practical aspects of spectroscopy including accuracy, precision, sources of error and procedure validation, • To determine metal content by atomic absorption spectrophotometry using the standard addition method, • To determine metal content by flame photometry using an internal standard.
Assessment Breakdown%
Course Work10.00%
Project10.00%
Practical30.00%
End of Module Formal Examination50.00%

Full Time

Course Work
Assessment Type Assessment Description Outcome addressed % of total Marks Out Of Pass Marks Assessment Date Duration
Continuous Assessment In class written evaluation of validation data. 5 10.00 0 0 Week 12 0
Project
Assessment Type Assessment Description Outcome addressed % of total Marks Out Of Pass Marks Assessment Date Duration
Group Project Using the problem-based learning technique, students (small group work) will be given a problem to solve in forensic/pharmaceutical/environmental science for which they should combine technical skills (developed in practical sessions) and theoretical knowledge (developed through classroom and self-directed learning) to define the problem and select and apply the most suitable technique to solve the problem and present their findings. 4 10.00 0 0 End-of-Semester 0
Practical
Assessment Type Assessment Description Outcome addressed % of total Marks Out Of Pass Marks Assessment Date Duration
Practical/Skills Evaluation A programme of weekly 3-hour laboratory practical sessions will run concurrently with lectures to reinforce fundamental theoretical concepts. Students will perform common analytical techniques using a suite of analytical instrumentation by following basic operating procedures, thereby gaining hands-on experience. Each student will be required to write a formal laboratory report for three experiments. 3 30.00 0 0 Every Week 0
End of Module Formal Examination
Assessment Type Assessment Description Outcome addressed % of total Marks Out Of Pass Marks Assessment Date Duration
Formal Exam End-of-Semester Final Examination 1,2 50.00 0 0 End-of-Semester 0
Reassessment Requirement
A repeat examination
Reassessment of this module will consist of a repeat examination. It is possible that there will also be a requirement to be reassessed in a coursework element.

DKIT reserves the right to alter the nature and timings of assessment

 

Module Workload & Resources

Workload: Full Time
Workload Type Workload Description Hours Frequency Average Weekly Learner Workload
Lecture Learning and Teaching Methods described in Module Content 3.00 Every Week 3.00
Practical A programme of weekly 3-hour laboratory practical sessions will run concurrently with lectures to reinforce fundamental theoretical concepts in separation science and spectroscopy. 3.00 Every Week 3.00
Directed Reading Lecture notes, Peer-reviewed papers, Textbooks, e-Resources 3.00 Every Week 3.00
Independent Study Independent/Group study 4.00 Every Week 4.00
Tutorial Problem solving, recap and revision tutorial. 1.00 Every Week 1.00
Total Weekly Learner Workload 14.00
Total Weekly Contact Hours 7.00
This course has no Part Time workload.
Resources
Recommended Book Resources
  • Harris D C 2012, Exploring chemical analysis, 5th Ed., WH Freeman
  • Skoog D. A., Holler F. J., and Crouch S.R. 2017, Principles of instrumental analysis, 7th Ed., Thomson Publ,
  • Harris, D.C. 2007, Quantitative chemical analysis, 7th Ed., WH Freeman
  • Watson D. G. 2012, Pharmaceutical analysis, 3rd Ed., Elsevier
Supplementary Book Resources
  • Sinead T. Loughran, Dermot Walls 2011, Protein Chromatography - Methods and Protocols; Methods in Molecular Biology, Humana Press Copies available from Sinead Loughran
  • De Levie, Robert 2001, How to Use Excel in Analytical Chemistry : And in General Scientific Data Analysis [electronic resource], Cambridge University Press DkIT Ebrary Collection
This module does not have any article/paper resources
Other Resources

Module Delivered in

Programme Code Programme Semester Delivery
Dk_NLBIO_8 Bachelor of Science (Honours) in Biopharmaceutical Science (2019) 3 Mandatory
DK_SENVI_8 Bachelor of Science (Honours) in Environmental Bioscience 3 Mandatory
DK_NLENV_8 Bachelor of Science (Honours) in Environmental Bioscience (2019) 3 Mandatory
DK_SAPBI_7 Bachelor of Science in Applied Bioscience 3 Mandatory
DK_NLBIO_7 Bachelor of Science in Bioscience (2019) 3 Mandatory
659 Bachelor of Science in Environmental Bioscience 3 Mandatory
DK_NLPHS_7 Bachelor of Science Pharmaceutical Science (2019) 3 Mandatory
Dk_SAPBI_6 Higher Certificate in Science 3 Mandatory
Dk_SPHAR_6 Higher Certificate in Science 3 Mandatory