Module Details

Module Code: INST S7Z02
Full Title: Analytical Science
Valid From:: Semester 1 - 2019/20 ( June 2019 )
Language of Instruction: 
Duration: 1 Semester
Credits:: 7.5
Module Owner:: Sinead Loughran
Departments: Unknown
Module Description: The aims of this module are to:
introduce students to selected aspects of modern chemical and biochemical analytical instrumentation and techniques; increase students' confidence in undertaking analysis (independently and in a team) and in designing and executing experiments; encourage students to take careful measurements to assess the validity of their results.
 
Module Learning Outcome
On successful completion of this module the learner will be able to:
# Module Learning Outcome Description
MLO1 Describe the fundamental theoretical basis of some chromatographic and spectroscopic including their applications and limitations.
MLO2 Compare and contrast common analytical techniques in separation science (GC and HPLC) and spectroscopy (Atomic and Molecular).
MLO3 Demonstrate proficiency in using a range of analytical instrumentation and techniques by following standard operating procedures.
MLO4 Combine technical skills and theoretical knowledge to define a problem in analytical science and select the most suitable technique to solve the problem.
MLO5 Assess the validity of analytical procedures and assays with reference to the ICH Harmonised Tripartite Guidelines on assay validation.
Pre-requisite learning
Module Recommendations
This is prior learning (or a practical skill) that is strongly recommended before enrolment in this module. You may enrol in this module if you have not acquired the recommended learning but you will have considerable difficulty in passing (i.e. achieving the learning outcomes of) the module. While the prior learning is expressed as named DkIT module(s) it also allows for learning (in another module or modules) which is equivalent to the learning specified in the named module(s).
No recommendations listed
 
Module Indicative Content
Chromatography
Introduction and general principles of chromatography, modes including Adsorption, Partition, Ion Exchange and Size Exclusion. Focus on gas liquid chromatography, including instrumentation, mobile/stationary phase selection, packed/capillary columns, detectors (FID, MS), applications and associated problems. Focus on HPLC; overview of HPLC, normal/reverse phase, column/mobile phase selection, diode-array detection and applications. Comparison of GC and HPLC. Chromatographic Parameters; Efficiency defined mathematically, Resolution defined and its dependence on chromatographic parameters will be illustrated for optimisation of resolution.
Molecular Spectroscopy
The electromagnetic spectrum, absorption of UV/visible radiation, excitation of electrons, relationship of structure to absorption, Beer-Lambert Law. The range of colorimeters and spectrophotometers and a survey of the main components parts. Applications of UV and visible spectroscopy and quantitative calculations.
Atomic Spectroscopy
Principle of absorption of energy by ground state atoms in gaseous state, electronic transitions, absorption/emission. Flame-based analytical systems; instrumentation, processes involved and examples of applications. Plasma-based techniques (ICP); comparison with flame-based techniques. Spectral, molecular, ionization and physical interferences. Quantitative techniques (including standard addition, internal standard). Application of atomic spectroscopy for sustainable environmental monitoring.
Validation of Analytical Procedures/Assays
Focus on ICH (International Conference on Harmonisation) Harmonised Tripartite Guidelines on assay validation, validation characteristics; accuracy, precision, specificity, detection limit, quantitation limit, linearity, range will be defined and equations will be used in practice to validate data obtained by students during laboratory practicals and (where available).
Learning and Teaching Methods
Teaching methods will comprise delivery of lectures and practical sessions with an emphasis on deep learning in a student-centred learning. A variety of blended and eLearning techniques will be deployed including in-class demonstrations, classroom assessment techniques, problem-based learning, peer assisted learning, self assessment and use of multi-media (animations, videos, eAssessments).
Virtual Learning Environment
The DkIT Virtual Learning Environment (Moodle) page for Analytical Science will be used extensively as a repository for lecture material, past exam papers, video links, online resource links, online quizzes, feedback, peer-reviewed articles as well as documents pertaining to practical lab sessions (Material Safety Data Sheets).
Virtual Laboratory Experiments
The use of virtual laboratory experiments in chromatography, spectroscopy and immobilisation reaction systems will complement laboratory practical sessions and reinforce fundamental theoretical concepts (for example; students will gain access to a CHROMacademy account managed by lecturer for full access to chromatography database, including HPLC, GC and hyphenated techniques, eLabs, assessments, webcasts, tutorials, lab simulations/ tools, peer-reviewed technical articles and application notes. Certification by CHROMacademy is also available to students upon completion of assessments).
Laboratory Practical Sessions
The following list is designed to serve as an illustration of possible practical exercises which would illustrate key concepts and techniques: • Separation of pigments by thin layer chromatography, • Use of gas liquid chromatography to identify and quantify components in a mixture, • Use of internal and external standards in gas liquid chromatography for quantification, • Use of high performance liquid chromatography to detect and quantify selected analytes, • To assess column quality in HPLC separations, • To verify Beer-Lambert Law in UV-Vis spectroscopy, • To study absorption of visible light by compounds and their absorption spectra, • To study practical aspects of spectroscopy including accuracy, precision, sources of error and procedure validation, • To determine metal content by atomic absorption spectrophotometry using the standard addition method, • To determine metal content by flame photometry using an internal standard.
Module Assessment
Assessment Breakdown%
Course Work10.00%
Project10.00%
Practical30.00%
Final Examination50.00%
Module Special Regulation
 

Assessments

Full Time On Campus

Course Work
Assessment Type Continuous Assessment % of Total Mark 10
Marks Out Of 0 Pass Mark 0
Timing S1 Week 12 Learning Outcome 5
Duration in minutes 0
Assessment Description
In class written evaluation of validation data.
Project
Assessment Type Group Project % of Total Mark 10
Marks Out Of 0 Pass Mark 0
Timing End-of-Semester Learning Outcome 4
Duration in minutes 0
Assessment Description
Using the problem-based learning technique, students (small group work) will be given a problem to solve in forensic/pharmaceutical/environmental science for which they should combine technical skills (developed in practical sessions) and theoretical knowledge (developed through classroom and self-directed learning) to define the problem and select and apply the most suitable technique to solve the problem and present their findings.
Practical
Assessment Type Practical/Skills Evaluation % of Total Mark 30
Marks Out Of 0 Pass Mark 0
Timing Every Week Learning Outcome 3
Duration in minutes 0
Assessment Description
A programme of weekly 3-hour laboratory practical sessions will run
concurrently with lectures to reinforce fundamental theoretical concepts.
Students will perform common analytical techniques using a suite of
analytical instrumentation by following basic operating procedures,
thereby gaining hands-on experience. Each student will be required to
write a formal laboratory report for three experiments.
Final Examination
Assessment Type Formal Exam % of Total Mark 50
Marks Out Of 0 Pass Mark 0
Timing End-of-Semester Learning Outcome 1,2
Duration in minutes 0
Assessment Description
End-of-Semester Final Examination
Reassessment Requirement
A repeat examination
Reassessment of this module will consist of a repeat examination. It is possible that there will also be a requirement to be reassessed in a coursework element.

DKIT reserves the right to alter the nature and timings of assessment

 

Module Workload

Workload: Full Time On Campus
Workload Type Contact Type Workload Description Frequency Average Weekly Learner Workload Hours
Lecture Contact Learning and Teaching Methods described in Module Content Every Week 3.00 3
Practical Contact A programme of weekly 3-hour laboratory practical sessions will run concurrently with lectures to reinforce fundamental theoretical concepts in separation science and spectroscopy. Every Week 3.00 3
Directed Reading Non Contact Lecture notes, Peer-reviewed papers, Textbooks, e-Resources Every Week 3.00 3
Independent Study Non Contact Independent/Group study Every Week 4.00 4
Tutorial Contact Problem solving, recap and revision tutorial. Every Week 1.00 1
Total Weekly Learner Workload 14.00
Total Weekly Contact Hours 7.00
This module has no Part Time On Campus workload.
 
Module Resources
Recommended Book Resources
  • Harris D C. (2012), Exploring chemical analysis, 5th. WH Freeman.
  • Skoog D. A., Holler F. J., and Crouch S.R.. (2017), Principles of instrumental analysis, 7th. Thomson Publ,.
  • Harris, D.C.. (2007), Quantitative chemical analysis, 7th. WH Freeman.
  • Watson D. G.. (2012), Pharmaceutical analysis, 3rd. Elsevier.
Supplementary Book Resources
  • Sinead T. Loughran, Dermot Walls. (2011), Protein Chromatography - Methods and Protocols; Methods in Molecular Biology, Humana Press, Copies available from Sinead Loughran.
  • De Levie, Robert. (2001), How to Use Excel in Analytical Chemistry : And in General Scientific Data Analysis [electronic resource], Cambridge University Press, DkIT Ebrary Collection.
This module does not have any article/paper resources
Other Resources