Dundalk Institute of Technology Programmes and Modules - BICH N8005 - Biochemistry 2

Module Details

Module Code:	BICH N8005
Full Title:	Biochemistry 2
Valid From::	Semester 1 - 2023/24 ( September 2023 )
Language of Instruction:	English

Duration:	1 Semester

Credits::	5

Module Owner::	Bernard Drumm Bernard Drumm

Departments:	Life and Health Sciences

Module Description:	Biochemistry 2 provides students with the biochemical basis of food metabolism on a cellular level. Students will learn the cellular pathways that enable the breakdown of carbohydrates, proteins and fats for energy and how also learn how excessive or reduced intake of certain biomolecules affects either the breakdown or storage of biomolecules in the body at a cellular level.

Module Learning Outcome
On successful completion of this module the learner will be able to:
#	Module Learning Outcome Description
MLO1	Summarize the pathways and identify the key cellular and organ locations for protein, carbohydrates and lipid metabolism.
MLO2	Outline how amino acids are obtained from ingestion of protein in the diet and explain the potential molecular fates of amino acids in anabolic and catabolic reactions, depending on the body’s needs.
MLO3	Describe key steps involved in the conversion of carbohydrates into usable ATP for metabolic processes in a cell and explain the dietary conditions under which carbohydrates might be stored or utilized to maintain normal blood glucose levels.
MLO4	Explain the metabolic pathways responsible for the digestion and utilization of lipids in fed vs fasted states.
MLO5	Perform experimental assays that enable qualitative & quantification of nutrition relevant biomolecules in various samples and experimentally demonstrate the impact of environmental stresses on biomolecule function.

Pre-requisite learning
Module Recommendations This is prior learning (or a practical skill) that is strongly recommended before enrolment in this module. You may enrol in this module if you have not acquired the recommended learning but you will have considerable difficulty in passing (i.e. achieving the learning outcomes of) the module. While the prior learning is expressed as named DkIT module(s) it also allows for learning (in another module or modules) which is equivalent to the learning specified in the named module(s).
No recommendations listed

Module Indicative Content
Protein To Amino Acid Metabolism Anatomical location of enzymatic protein breakdown in digestive system, activation of proteolytic enzymes in stomach, pancreatic zymogen activation cascade from pancreas to small intestine, importance of endopeptidases and exopeptidases in polypeptide breakdown.
Amino Acid Metabolism Absorption of amino acids in small intestine, replenishment of depletion of amino acid pools, amino acids in anabolic reactions via dehydration synthesis, conversion of amino acids via transamination, amino acid deamination in the liver, conversion of amino acids to urea, utilization of amino acids in carbohydrate metabolism.
Complex Carbohydrate Metabolism Location of major enzymatic pathways involved in polysaccharide digestion to simple monosaccharides, monosaccharide absorption in the small intestine, transport and regulation of systemic glucose via glycogen stores in liver and muscle (glycogenesis, glycogenolysis, gluconeogenesis).
Cellular Metabolism of Glucose Aerobic and anaerobic breakdown and utilization of glucose via glycolysis, citric acid cycle, electron chain transport system.
Lipid Metabolism Lipid digestion in the small intestine and stomach, secretion of liver bile salts for lipid emulsification and absorption in small intestine, fat utilization and generation via lipolysis, lipogenesis, beta oxidation and ketosis, fed vs fasted metabolism.
Indicative Practical Content Investigation of biomolecular solubility and hydrophobicity. Identification of biomolecules (proteins carbohydrates, lipids) in unknown samples (Molisch Test, Iodine Test, Benedicts Assay, Barfoeds Test, Seliwan Test, Bials Test, Sudan Red Test). Quantification of protein concentrations in various food items (Biuret / Bradford assay). Quantification of reducing sugar concentrations in various food items (DNS assay). Determination of environmental stresses (pH and temperature) on biomolecule stability.

Assessment Breakdown	%
Module Assessment
Course Work	30.00%
Practical	30.00%
Final Examination	40.00%

Module Special Regulation

Assessments

Full-time

Course Work

Assessment Type	Open-book Examination	% of Total Mark	30
Marks Out Of	100	Pass Mark	0
Timing	S2 Week 26	Learning Outcome	1,2,3
Duration in minutes	0
Assessment Description There will be a 60-minute, online open book assessment on Moodle where students will be free to access materials to assist them answer application style problem sets and exercises. This assessment will assist students in evaluating their understanding of the key module material half-way through the semester.

No Project

Practical

Assessment Type	Practical/Skills Evaluation	% of Total Mark	30
Marks Out Of	100	Pass Mark	0
Timing	Every Week	Learning Outcome	5
Duration in minutes	0
Assessment Description During each weekly 2-hour practical session students will complete a laboratory workbook, documenting their results and providing them with opportunities to create and analysis data in the form of tables, graphs and also enable troubleshooting and assess ability to critically evaluate data and concepts. This will provide the student with the opportunity to back up the theory covered in formal lectures with practical experience.

Final Examination

Assessment Type	Formal Exam	% of Total Mark	40
Marks Out Of	100	Pass Mark	0
Timing	End-of-Semester	Learning Outcome	1,2,3,4
Duration in minutes	120
Assessment Description End-of-semester final examination consisting of MCQs, short answer questions and application style questions.

Reassessment Requirement
A repeat examination Reassessment of this module will consist of a repeat examination. It is possible that there will also be a requirement to be reassessed in a coursework element.

DKIT reserves the right to alter the nature and timings of assessment

Module Workload

Workload: Full-time
Workload Type	Contact Type	Workload Description	Frequency	Average Weekly Learner Workload	Hours
Lecture	Contact	No Description	Every Week	2.00	2
Practical	Contact	No Description	Every Second Week	1.00	2
Lecturer-Supervised Learning (Contact)	Contact	Application class where students will use knowledge acquired through lectures and outside reading to solve problem sets and work through team exercises.	Every Week	1.00	1
Independent Study	Non Contact	Students will be expected to review lecture material and seek outside sources (both online and hard copy) for reading and revisions. Useful video links and paper resources for these independent hours will be provided by lecturer.	Every Week	2.00	2
Total Weekly Learner Workload					6.00
Total Weekly Contact Hours					4.00

This module has no Part-time workload.

This module does not have any book resources
Module Resources
This module does not have any article/paper resources
This module does not have any other resources